Isolation of papain-soluble collagen from the skin of snake-head fish (Channa striata)
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Isolation of papain-soluble collagen from the skin of snake-head fish (Channa striata)

Articles
https://doi.org/10.20956/canrea.v3i2.367
Published 2020-12-30
Andi Rahmayanti Ramli
Department of Agriculture Technology, Faculty of Agriculture, Hasanuddin University, Indonesia
Andi Rezky Annisa
Study Program of Aquatic Resources Management, Faculty of Marine and Fisheries, Hasanuddin University, Indonesia
Nur Alim Bahmid
Food Quality and Design Group, Wageningen University and Research, Netherlands
Muhammad Dalvi Mustafa
Study Program of Social Economic of Fisheries, Faculty of Marine and Fisheries, Hasanuddin University, Indonesia
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Keywords

Collagen
Papain
Snake-head fish

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How to Cite

Ramli, A. R., Annisa, A. R. ., Bahmid, N. A., & Mustafa, M. D. . (2020). Isolation of papain-soluble collagen from the skin of snake-head fish (Channa striata). Canrea Journal: Food Technology, Nutritions, and Culinary Journal, 3(2), 87–93. https://doi.org/10.20956/canrea.v3i2.367
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Abstract

Snake-head fish (Channa striata) skin categorized a byproduct contains a higher concentration of collagen. The collagen can be extracted by using protease, which is known as Papain Soluble Collagen (PaSC). This study aimed to isolate the collagen from the snake-head fish skin using papain. The yield percentage and moisture content of PaSC in the skin was determined. Measurements using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Fourier transform infrared spectroscopy (FTIR) were performed to characterize the PaSC and its functional groups. The results showed that the yields and moisture content of PaSC were 8.9% and 6.07%, respectively. The PaSC characterized by two α-chains appeared as collagen type I. Fourier transform infrared (FTIR) spectra of PaSC confirmed a triple-helical structure of collagen. The results indicated that snake-head fish skin could be used as potential resources of collagen and papain can be used as an alternative affordable enzyme.

https://doi.org/10.20956/canrea.v3i2.367
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