ANALISIS SECARA SIMULTAN BEBERAPA CAMPURAN PEMANIS PADA PRODUK MINUMAN RINGAN DENGAN METODE KROMATOGRAFI CAIR KINERJA TINGGI
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Abstrak
Minuman ringan umumnya selalu mengandung pemanis, sehingga perlu adanya pengembangan metoda untuk mengukur kadar pemanis dalam minuman ringan. Beberapa penelitian menunjukkan bahwa penggunaan pemanis dapat memicu timbulnya berbagai macam penyakit sehingga perlu dilakukan pengawasan terhadap penggunaanya pada minum ringan. Penelitian ini bertujuan untuk mengembangkan metode analisis dan mengetahui kadar asesulfam, natrium sakarin dan aspartam pada sampel minuman ringan di kota Medan. Penelitian ini bersifat eksperimental deskriptif dengan menggunakan metode KCKT fase terbalik dengan kolom Eclipse plus C18 250 x 4,6 mm, 5 μm, fase gerak buffer fosfat pH 5 : metanol (70 : 30), laju alir 1,0 mL/menit. Hasil validasi metode menunjukkan uji linearitas telah memenuhi syarat dengan koefisien determinasi (R2) yaitu sebesar 0,99998 - 0,99999. Nilai LOD berkisar antara 0,0482 hingga 0,2482 mg/kg. LOQ berkisar antara 0,1605 hingga 0,8273 mg/kg, dan nilai persen RSD keterulangan berkisar 0,49% -0,91%, dan presisi antara metode 0,24% -0,63%. Hasil persen recoverynya rata-rata sebesar 93,59% – 105,28%. Metode ini telah berhasil diterapkan pada penentuan kadar pemanis pada produk minuman ringan.
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Cara Mengutip
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