KARAKTERISASI ENZIM PEKTINASE YANG DIHASILKAN DARI ISOLAT KHAMIR INDIGENUS YANG DIISOLASI SELAMA FERMENTASI BIJI KOPI ROBUSTA

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Published Apr 22, 2025
https://doi.org/10.70124/at.vi.1372
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Jurnal Agritechno Vol. 18, Nomor 1, April 2025

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Hasanul Fitri Fitri
a:1:{s:5:"en_US";s:27:"Universitas Negeri Makassar";}
Subari Yanto
Universitas Negeri Makassar
Reski Praja Putra
Universitas Negeri Makassar

Abstract

The following research aims to understand indigenous yeast isolates that are pectinolytic, the effect of incubation time on pectinase enzyme activity, and the pH and temperature properties of pectinase enzymes obtained from indigenous yeast resulting from spontaneous fermentation of robusta coffee beans. The following type of research is experimentation through RAL (Completely Randomized Design). This research first carried out selection of pectinolytic yeast, then determined the optimum incubation duration, pH and optimum temperature of the pectinase enzyme. The yeast isolate used was selected using media (1% pectin + 0.1% Congo red + 2% agar) to determine the highest pectinolytic ability. Selected pectinolytic yeast isolates will proceed to the stage of determining the optimum incubation time with periods of 24, 48, 72, 96, 120, and 144 hours, where every 24 hours enzyme extraction is carried out. Furthermore, the crude extract of the pectinase enzyme produced from selected pectinolytic yeast isolates was characterized at pH (3, 3.5, 4, 4.5, 5, 5.5, 6) and temperature (30oC, 40oC, 50oC, 60oC, 70oC) to determine the optimum pH and temperature. The research results showed that tests on seven indigenous yeast isolates were pectinolytic and the one with the highest pectinolytic ability was isolate K24I7 with a clear zone diameter of 3.13 mm. In determining the optimum incubation time, it was found that at an incubation time of 24 hours, isolate K24I7 was able to produce enzyme activity that was similar to other incubation times with a specific enzyme activity of 10.08 U/mg. The results of the characterization of the pectinase enzyme produced from isolate K24I7 were optimum at pH 5.5 with a specific enzyme activity value of 10.09 U/mg and an optimum temperature in the range of 40oC-60oC with an average specific enzyme activity of 10.08 U/mg. The pectinase enzyme produced from isolate K24I7 is thermophilic because it is still active at 70oC.

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How to Cite
Fitri, H. F., Yanto, S., & Praja Putra, R. (2025). KARAKTERISASI ENZIM PEKTINASE YANG DIHASILKAN DARI ISOLAT KHAMIR INDIGENUS YANG DIISOLASI SELAMA FERMENTASI BIJI KOPI ROBUSTA. Jurnal Agritechno, 18(1), 27–38. https://doi.org/10.70124/at.vi.1372
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References

  1. Angraini, I., Rejeki, S. F., & Endang, K. 2019. Isolasi Khamir Fermentatif dari Batang Tanaman Tebu (Saccharum officinarum. L) dan Hasil Identifikasinya Berdasarkan Sekuens Internal Transcribed Spacer. Berkala Bioteknologi, Vol. 2, No.2
  2. Antoni, H. 2016. Fermentasi Spontan Bekasam Ikan Nila (Oreochromis niloticus) Menggunakan Kerak Nasi Kering. Departemen Teknologi Hasil Perairan Fakultas Perikanan Dan Ilmu Kelautan. Skripsi. Institut Pertanian Bogor : Bogor
  3. Aryani, S. W. 2012. Isolasi dan Karakterisasi Ekstrak Kasar Enzim Selulase dari Kapang Selulolitik Mucor sp.B2. Skripsi. Surabaya: Fakultas Sains dan Teknologi Universitas Airlangga
  4. Boyd, A. R., Gunasekera, T. S., Attfield, P. V., Simic, K., Vincent, S. F., & Veal, D. A. 2003. A Flow-Cytometric Method For Determination Of Yeast Viability And Cell Number In A Brewery. FEMS Yeast Research 3
  5. Fransistika, R., Nora, I., & Lia, D. 2012. Pengaruh Waktu Fermentasi Campuran Trichoderma reesei dan Aspergillus niger Terhadap Kandungan Protein dan Serat Kasar Ampas Sagu. JKK, Vol 1 (1) : 35-39
  6. Glazer, A. N., & Nikaido, H. 2007. Microbial Biotechnology: Fundamental of Applied Microbiology. Ed ke-2. Cambridge: Cambridge Univ Pr
  7. Hardianto., Anton, M., & Antok, W.S. 2018. Optimalisasi Fosfat untuk Meningkatkan Pertumbuhan Kerapatan Populasi dan Kemampuan Antagonis Saccharomyces cerevisiae terhadap Fusarium sp. Jurnal Sains dan Teknologi. Vol. 10 No.2 : 27-41
  8. Haq, H., M.A. Ashaf & J. Qadeer. 2010. Pearl millet, a source of α-amylase production by Bacillus licheniformis. Bioresour. Technol., 96:1201-1204
  9. Herdyastuti, N., Raharjo, T. J., Mudasir, & Matsjeh. S. 2009. Chitinase and Chitinolytic Microorganism: Isolation, Characterization, and Potential. Indo J. Chem. 9 (1): 37-47
  10. Hikmah, A. N. 2021. Isolasi dan Identifikasi Khamir Indigenus pada Fermentasi Spontan Biji Kopi Robusta (Coffea canephora) Asal Kabupaten Bantaeng. Skripsi. Program Studi Pendidikan Teknologi Pertanian Fakultas Teknik Universitas Negeri Makassar. Makassar
  11. Kusiyanto, G., Purwatiningsih., & Kahar, M. 2019. Skrining dan Identifikasi Bakteri Pektinolitik Endosembion dalam sistem Pencernaan serangga Penggerek Kopi (Hypothenemus hampei Ferr.). Journal Of Trofical Biology, Vol. 7. No. 2
  12. Lahninger, A. L. 1997. Dasar-Dasar Biokimia. Thenawidjaja M, penerjemah. Jakarta : Erlangga. Terjemahan dari : Principles of Biochemistry
  13. Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. 1951. Protein Measurement Whit The Folin Phenol Reagent, J. Biol. Chem, 193: 265-275
  14. Mashitoh, Erna. 2012. Pengaruh Konsentrasi Sukrosa Terhadap Pertumbuhan Khamir Roti Saccharomyces cerevisiae pada Media Bekatul dalam Produksi Protein Sel Tunggal. Skripsi. Universitas Sebelas Maret Surakarta
  15. Mulyani, N. S., Asy’ari, M., & Prasetyoningsih, H. 2009. Penentuan Konsentrasi Optimum Oat Spelt Xylan Pada Produksi Xilanase dari Aspergillus Niger dalam Media PDB (Potato Dextrose Borth). Jurnal Kimia Sains dan Apl. 12 (1) : 6-8
  16. Oktavia, Y., Andhikawati, A., Nurhayati, T., & Tarman, K. 2014. Karakterisasi Enzim Kasar Selulase Kapang Endofit dari Lamun. Jurnal Ilmu dan Teknologi Kelautan Tropis. 6 (1) : 209-218
  17. Purkan., Purnama, H. D., & Sumarsih, S. 2015. Produksi Enzim Selulase dari Aspergillus niger Menggunakan Sekam Padi dan Ampas Tebu sebagai Induser. Jurnal ilmu dasar. 16 (2): 95-102
  18. Safaria, S., Idiawati, N., & Zaharah, T. A. 2013, Efektivitas Campuran Enzim Selulase dari Aspergillusniger niger dan Trichoderma reesei dalam Menghidrolisis Substrat Sabut Kelapa, JKK, 2 (1), 46-51
  19. Safitri, R., & S. S. Novel. 2013. Medium Analisis Mikrorganisme (Isolasi dan Kultur)., Jakarta : Trans Info Media. p. 29-34
  20. Sieiro, C., Fraga1, B.G., Seijas1, J.L., Silva1, A.F., & Villa, T.G. 2012. Microbial Pectic Enzymes in the Food and Wine Industry. Food Industrial Processes Methods and Equipment
  21. Siska, F., & Astuti, W. 2018. Isolasi dan Penentuan Kondisi Kerja Optimum Amilase dari Rebung Bambu Serit (Gigantochloa robusta Kurz.). Kimia FMIPA UNMUL
  22. Spencer, J.F.T., & D.M. Spencer. 1997. Yeasts in Natural and Artificial Habitats. Berlin: Springer Verlag
  23. Sumardjo, Darmin. 2009. Pengantar Kimia : Buku Panduan Kuliah Mahasiswa Kedokteran dan Program Strata I Fakultas Bioeksakta. Jakarta: EGC
  24. Suryaningsih, V., Rejeki, S.F., & Endang, K. 2018. Karakteristik Morfologi, Biokimia, dan Molekuler Isolat Khamir IK-2 Hasil Isolasi dari Jus Buah Sirsak (Annona muricata L.). Jurnal Biologi. Vol 7 No. 1
  25. Triana, R. 2013. Pemurnian dan Karakterisasi Enzim Glukosa Oksidase dari Isolat Lokal Aspergillus niger (IPBCC.08.610). Skripsi. Departemen Biokimia-FMIPA, Institut Pertanian Bogor
  26. Wuryanti. 2003. Penentuan aktivitasspesifik heksokinase dari limbah anggur pisangbiji. JSKA 7(3) : 2,4
  27. Yopi., Nanik, R., Ade, A., Fitria, D., & Anja, M. 2013. Purifikasi dan Karakterisasi Enzim pektinase dari Aspergillus utus BL5. J. Berita Biologi. 12 (3)