Article Sidebar

Published: 2026-06-30
Keywords:
Bitter leaf , Neem , Postharvest loss , Shelf life , Tomato
Section: Articles
Folasade Ade-Ogunnowo  Department of Biological Sciences, Tai Solarin Federal University of Education, Ijagun, Ogun State , Nigeria
Oluseyi  Department of Biological Sciences, Tai Solarin Federal University of Education, Ijagun, Ogun State , Nigeria
Abstract:

Postharvest weight loss is a major constraint on tomato (Solanum lycopersicum L.) shelf life in tropical environments. This study evaluated ethanol and aqueous leaf extracts of Vernonia amygdalina (bitter leaf) and Azadirachta indica (neem) for their effects on weight retention and storage kinetics of San Marzano and Royal tomato cultivars over 20 days at ambient conditions (28 ± 2°C). Treatments comprised ethanol extracts (50–200 g/500 mL), aqueous hot and cold extracts (50 g/500 mL), and a distilled water control in a completely randomised design. Physiological loss in weight (PLW) differed significantly among treatments (p < 0.05). The 100 g/500 mL ethanol extract of bitter leaf gave the lowest PLW and highest weight retention, in Royal (37.3%) followed by San Marzano (41.9%). Hot aqueous extracts showed moderate efficacy (46.2–50.3% PLW), cold aqueous extracts were least effective (70.7–77.3%), and excess ethanol (150–200 g/500 mL) accelerated deterioration (81.6–86.9% PLW), indicating phytotoxicity. Weight loss followed an exponential decay pattern (R² = 0.91–0.98), with lower decay constants in optimised treatments (k ≈ 0.063–0.069 day⁻¹). Royal consistently showed greater postharvest stability than San Marzano. These findings indicate that a 100 g/500 mL V. amygdalina ethanol formulation offers an accessible, low-cost postharvest coating that can extend tomato shelf life and reduce reliance on synthetic fungicides for smallholder farmers in tropical regions.

Abdulrahman, I. M., Umar, U. A., & Oyedeji, A. N. (2025). Postharvest losses of a developing nation's tomatoes value chain-evidence from actors' perspectives in Nigeria. J. Appl. Res. Ind. Eng., 12(1), 119–132. https://doi.org/10.22105/jarie.2024.422732.1570

Ajenifujah-Solebo, S. O., Akin-Idowu, P. E., Aduloju, A. O., Adedeji, V. O., Akinyode, E. T., Ibitoye, D. O., ... & Bello, F. (2025). Tomato crop improvement efforts in Nigeria: Past, current and future perspectives. Solanum lycopersicum L.-Res. Methods, Approaches, Perspect., 29. https://doi.org/10.5772/intechopen.1009299

Alara, O. R., Abdurahman, N. H., & Olalere, O. A. (2020). Ethanolic extraction of flavonoids, phenolics and antioxidants from Vernonia amygdalina leaf using two-level factorial design. Journal of King Saud University-Science, 32(1), 7-16. https://doi.org/10.1016/j.jksus.2017.08.001

Ali, J., Hussain, A., & Abbas, J. (2025). In vitro control of post-harvest fruits rot pathogenic fungi using Azadirachta indica (Neem) seeds and leaves extracts. Bull. Biol. Allied Sci. Res., 2025(1), 102–102. https://doi.org/10.64013/bbasr.v2025i1.102

Anusha, M., Sri, K. R., Lokesh, M., Deepthi, N. M., & Malleswari, M. D. (2024). Postharvest management techniques for improved shelf life of horticultural crops: A review. J. Exp. Agric. Int., 46(11), 362–380. https://doi.org/10.9734/jeai/2024/v46i113059

Azwanida, N. N. (2015). A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med. Aromat. Plants, 4, 2167-0412. https://doi.org/10.4172/2167-0412.1000196

Barraj Barraj, R., Segado, P., Moreno-González, R., Heredia, A., Fernández-Muñoz, R., & Domínguez, E. (2021). Genome-wide QTL analysis of tomato fruit cuticle deposition and composition. Hortic. Res., 8. https://doi.org/10.1038/s41438-021-00548-5

Benito-Román, Ó., Blanco, B., Sanz, M. T., & Beltrán, S. (2020). Subcritical water extraction of phenolic compounds from onion skin wastes (Allium cepa cv. Horcal). Antioxidants, 9(12), 1233. https://doi.org/10.3390/antiox9121233

Chen, D., Liu, L., Gao, Z., Zhao, J., Yang, Y., & Shen, Z. (2025). Preservation of fruit quality at postharvest through plant-based extracts and elicitors. Horticulturae, 11(10), 1186. https://doi.org/10.3390/horticulturae11101186

Chime, A. O., & Aiwansoba, R. O. (2023). Antifungal activity of neem (Azadirachta indica) leaf extract against pathogens associated with tomato fruit spoilage. Afr. Sci., 24, 297–303

Darwin, R., Valmon, R., Chithanna, S., Galla, S. H., Syed, S. H., Mohathasim Billah, A. A., ... & Arjun, U. V. N. V. (2025). Sustainable extraction and purification of phytochemicals: A review of green solvents and techniques. Chem. Methodol., 9(5), 356–385. https://doi.org/10.48309/chemm.2025.504050.1892

Dheeba, B., Niranjana, R., Sampathkumar, P., Kannan, K., & Kannan, M. (2015). Efficacy of neem (Azadirachta indica) and tulsi (Ocimum sanctum) leaf extracts against early blight of tomato. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 85(1), 327-336. https://doi.org/10.1007/s40011-014-0340-9

Do, Q. D., Angkawijaya, A. E., Tran-Nguyen, P. L., Huynh, L. H., Soetaredjo, F. E., Ismadji, S., & Ju, Y. H. (2014). Effect of extraction solvent on total phenol content and antioxidant activity. J. Food Drug Anal., 22(3), 296–302. https://doi.org/10.1016/j.jfda.2013.11.001

Edo, G. I., Samuel, P. O., Jikah, A. N., Onoharigho, F. O., Idu, L. I., Obasohan, P., ... & Owigho, J. E. (2023). Biological and bioactive components of bitter leaf. Food Chem. Adv., 3, 100488. https://doi.org/10.1016/j.focha.2023.100488

Ekhuemelo, C., & Eigege, O. (2017). Evaluation of stem bark extracts of Azadiratcha indica and Vernonia amygdalina. Asian Res. J. Agric., 5(3), 1–7. https://doi.org/10.9734/ARJA/2017/34640

El Alami El Hassani, N., Baraket, A., & Alem, C. (2025). Recent advances in natural food preservatives. J. Food Meas. Charact., 19(1), 293–315. https://doi.org/10.1007/s11694-024-02969-x

El-Saadony, M. T., Saad, A. M., Mohammed, D. M., Alkafaas, S. S., Abd El-Mageed, T. A., Fahmy, M. A., ... & El-Tarabily, K. A. (2025). Plant bioactive compounds: A comprehensive review. Front. Nutr., 12, 1659743. https://doi.org/10.3389/fnut.2025.1659743

Firdous, N., Farooq, U., Batool, A., Aslam, A., Erum, A., Ahmad, S., ... & Khalil, S. (2025). Postharvest metabolomics of tomato. In Omics Approaches for Tomato Yield and Quality Trait Improvement, 147–176. Springer. https://doi.org/10.1007/978-981-96-3890-1_7

Fujita, M., & Hasanuzzaman, M. (2022). Approaches to enhancing antioxidant defense in plants. Antioxidants, 11(5), 925. https://doi.org/10.3390/antiox11050925

Ghamba, P. E., Balla, H., Goje, L. J., Halidu, A., & Dauda, M. D. (2014). In vitro antimicrobial activities of Vernonia amygdalina on selected clinical isolates. International Journal of Current Microbiology and Applied Sciences, 3(4), 1103-1113.

Godana, E. A., & Gurmu, A. H. (2020). Postharvest disease control approaches. Ann. Food Sci. Technol., 21(3)

Hosea, Z. Y., Liamngee, K., Owoicho, A. L., & David, T. (2017). Neem leaf powder and tomato shelf life. Int. J. Dev. Sustain., 6(10), 1334–1349

Howlader, N. C., Hossain, M. M., Roy, T. K., Ahsan, S. M., Injamum-Ul-Hoque, M., & Miah, M. H. (2026). Botanical extracts for postharvest preservation. J. Crop Health, 78(1), 17. https://doi.org/10.1007/s10343-025-01277-8

Jung, J. M., Shim, J. Y., Chung, S. O., Hwang, Y. S., Lee, W. H., & Lee, H. (2019). Changes in quality parameters of tomatoes during storage: A review. Korean J. Agric. Sci., 46(2), 239–256. https://doi.org/10.7744/kjoas.20190011

Kitinoja, L., Odeyemi, O., Dubey, N., Musanase, S., & Gill, G. S. (2019). Commodity system assessment studies on the postharvest handling and marketing of tomatoes in Nigeria, Rwanda and Maharashtra, India. J. Hortic. Postharvest Res., 2(Special Issue), 1–14. https://doi.org/10.22077/jhpr.2019.2060.1040

Kocira, A., Kozłowicz, K., Panasiewicz, K., Staniak, M., Szpunar-Krok, E., & Hortyńska, P. (2021). Polysaccharides as edible films and coatings: Characteristics and influence on fruit and vegetable quality—A review. Agronomy, 11(5), 813. https://doi.org/10.3390/agronomy11050813

Kumar, A., Kumar, V., Gull, A., & Nayik, G. A. (2020). Tomato antioxidants. In Antioxidants in Vegetables and Nuts, 191–207. Springer. https://doi.org/10.1007/978-981-15-7470-2_10

Kumar, M., Chandran, D., Tomar, M., Bhuyan, D. J., Grasso, S., Sá, A. G. A., ... & Mekhemar, M. (2022). Tomato seed nutraceutical properties. Horticulturae, 8(3), 265. https://doi.org/10.3390/horticulturae8030265

Lara, I., Belge, B., & Goulao, L. F. (2014). The fruit cuticle as a modulator of postharvest quality. Postharvest Biol. Technol., 87, 103–112. https://doi.org/10.1016/j.postharvbio.2013.08.012

Nursuhaili, A. B., Nur Afiqah Syahirah, P., Martini, M. Y., Azizah, M., & Mahmud, T. M. M. (2019). A review: medicinal values, agronomic practices and postharvest handlings of Vernonia amygdalina. Food Res., 3(5), 380–390. https://doi.org/10.26656/fr.2017.3(5).306

Nxumalo, K. A., Aremu, A. O., & Fawole, O. A. (2021). Potentials of medicinal plant extracts as an alternative to synthetic chemicals in postharvest protection and preservation of horticultural crops: A review. Sustainability, 13(11), 5897. https://doi.org/10.3390/su13115897

Odeyemi, O. M., Kitinoja, L., Dubey, N., Musanase, S., & Gill, G. S. (2022). Preliminary study on improved postharvest practices for tomato loss reduction in Nigeria, Rwanda and India. Afr. J. Sci. Technol. Innov. Dev., 14(6), 1500–1505. https://doi.org/10.1080/20421338.2021.1961986

Okolo, J. C., Igborgbor, J. C., Eze, E. M., Ogu, G. I., & Jonah, G. U. (2022). The shelf life of tomato fruits (Solanum lycopersicum L.) treated with extracts of two medicinal plants: Azadirachta indica and Vernonia amygdalina. Int. J. Environ., 11(2), 124–140. https://doi.org/10.3126/ije.v11i2.48653

Patathananone, S., Pothiwan, M., Uapipatanakul, B., & Kunu, W. (2023). Inhibitory effects of Vernonia amygdalina leaf extracts on free radical scavenging, tyrosinase, and amylase activities. Prev. Nutr. Food Sci., 28(3), 302–311. https://doi.org/10.3746/pnf.2023.28.3.302

Peleg, M. (2019). Food degradation kinetics. Food Eng. Rev., 11(1), 1–13. https://doi.org/10.1007/s12393-018-9185-y

Salgado-Cruz, M. D. L. P., Salgado-Cruz, J., García-Hernández, A. B., Calderón-Domínguez, G., Gómez-Viquez, H., Oliver-Espinoza, R., Fernández-Martínez, M. C., & Yáñez-Fernández, J. (2021). Chitosan as a coating for biocontrol in postharvest products: A bibliometric review. Membranes, 11(6), 421. https://doi.org/10.3390/membranes11060421

Shahbaz, M. U., Arshad, M., Mukhtar, K., Nabi, B. G., Goksen, G., Starowicz, M., Nawaz, A., Ahmad, I., Walayat, N., Manzoor, M. F., & Aadil, R. M. (2022). Natural plant extracts: An update about novel spraying as an alternative of chemical pesticides to extend the postharvest shelf life of fruits and vegetables. Molecules, 27(16), 5152. https://doi.org/10.3390/molecules27165152

Sibomana, M. S., Workneh, T. S., & Audain, K. J. F. S. (2016). Tomato supply chain losses. Food Security, 8(2), 389–404. https://doi.org/10.1007/s12571-016-0562-1

Taoukis, P., Labuza, T., & Saguy, I. (1997). Food deterioration kinetics. In Handbook of Food Engineering Practice, 361–404

Tripathi, A., Chandrasekaran, N., Raichur, A. M., & Mukherjee, A. (2009). Antibacterial applications of silver nanoparticles synthesized by aqueous extract of Azadirachta indica (Neem) leaves. Journal of Biomedical Nanotechnology, 5(1), 93-98. https://doi.org/10.1166/jbn.2009.038

Tušek, A. J., Benković, M., Valinger, D., Jurina, T., Belščak-Cvitanović, A., & Kljusurić, J. G. (2018). Bioactive extraction modelling. Ind. Crops Prod., 126, 449–458. https://doi.org/10.1016/j.indcrop.2018.10.040

Umeohia, U. E., & Olapade, A. A. (2024). Tomato postharvest technologies. Am. J. Food Sci. Technol., 12(2), 42–64. https://doi.org/10.12691/ajfst-12-2-1

Wylie, M. R., & Merrell, D. S. (2022). The antimicrobial potential of the neem tree Azadirachta indica. Front. Pharmacol., 13, 891535. https://doi.org/10.3389/fphar.2022.891535

Xie, L., Guerra, M., Tijskens, L. M. M., Schoevaars, A. M., Verdonk, J. C., Woltering, E. J., & Schouten, R. E. (2025). Tomato weight loss modelling. Postharvest Biol. Technol., 230, 113747. https://doi.org/10.1016/j.postharvbio.2025.113747

Žagar, T., Frlan, R., & Kočevar Glavač, N. (2024). Using subcritical water to obtain polyphenol-rich extracts with antimicrobial properties. Antibiotics, 13(4), 334. https://doi.org/10.3390/antibiotics13040334

Folasade Ade-Ogunnowo adeogunnowo@tasued.edu.ng

Department of Biological Sciences, Tai Solarin Federal University of Education, Ijagun, Ogun State

Nigeria

Oluseyi

Department of Biological Sciences, Tai Solarin Federal University of Education, Ijagun, Ogun State

Nigeria

Effects of Vernonia amygdalina and Azadirachta indica Extracts on Postharvest Weight Loss and Shelf Life of Tomato (Solanum lycopersicum L.) Cultivars. (2026). Salaga Journal, 4(1), 16-26. https://doi.org/10.70124/salaga.v4i1.2286